Fig. 3
From: Arginase-I enhances vascular endothelial inflammation and senescence through eNOS-uncoupling
The antioxidant NAC significantly blunted senescence and inflammation caused by Arg-I overexpression in endothelial cells. Non-senescent endothelial cells were transduced and treated with NAC as described in Fig. 2b. a Immunoblotting analysis of endothelial inflammation markers VCAM-1 and ICAM-1 expression, and senescence markers p53-S15 and p21Cip1 levels. Tubulin served as loading control. Lysates were prepared 64 h post-transduction with serum-starvation for the last 16 h. b SA-β-gal staining on day 5 post-transduction. Bar graphs show quantification of relative of SA-β-gal positive cells. c Monocytes adhesion assay. Arg-I overexpressing HUVECs were treated with or without NAC (5 mmol/L, 64 h) after transduction. CFDA-SE fluorescence labeled THP-1 monocytes were then added to HUVECs. After washing, adhesion of the labeled monocytes to endothelial cells was evaluated. Bar graphs in the right or lower panels show quantification of the corresponding signals. ***p < 0.005 vs. control (con). #p < 0.05, ##p < 0.01 vs. Arg-I. Scale bar 0.2 mm