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Fig. 3 | BMC Research Notes

Fig. 3

From: MicroRNA expression profiles in human CD3+ T cells following stimulation with anti-human CD3 antibodies

Fig. 3

Effects of anti-CD3 stimulation on the expression of genes involved in CD4+ T cell differentiation. CD3+ T cells were isolated 72 h post stimulation, and mRNA was analyzed by qPCR to determine the expression levels of a TBX21 and STAT4, b GATA3, c RORγt and STAT3, and d FOXP3 and GITR. The data were normalized to the expression levels of the same genes in CD3+ T cells from untreated cultures. Representative results from three independent experiments are shown (n = 5; p < 0.05). B2M was used as an internal control for data normalization

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