Fig. 2

Flowcytometric analysis of CD4+ T cells of blood and CSF after stimulation with TB antigens. The gating strategy excluded debris and identified CD4+ on CD45+ lymphocytes. The subsequent analysis was on CD4+ gate to describe IFN-γ, IL-2, and TNF-α total producing T cells. At the bottom the percentages of the different population cells, calculated by FlowJo Software version 7.6.5 were showed and were defined in CD4+ cell gate on the basis of total IFN-γ, IL-2, and TNF-α producing. T cells producing any of the 3 cytokines (IFN-γ or IL-2 or TNF-α) were defined as “activated T cells”