Fig. 3
From: RNA isolation from precision-cut lung slices (PCLS) from different species
RTqPCR as a follow-up endpoint of RNA from human PCLS. a Efficiency calculation for B2M primer using several A549 cDNA dilutions. b Efficiency analysis for different RNA preparations from A549 cells and human PCLS (different donors). Results represent the means of at least three samples (RINÂ >Â 7.0). c MUC5AC expression in human PCLS as an example of lung-specific gene expression (calibration curve, melting curve, gel image)