Skip to main content

Table 2 RNA isolation from PCLS from different species using an optimized protocol

From: RNA isolation from precision-cut lung slices (PCLS) from different species

Species Incu-bation period RNA yield (µg) Absorbance 260/280 RIN value
Mean Min. Mean Min. p value Mean Min. p value
Human (n = 12) w/o 1.12 ± 0.44 0.62 1.91 ± 0.07 1.79   8.9 ± 0.7 7.8  
Human, donor 1 (n = 12) 3 days 2.34 ± 1.03 1.34 1.90 ± 0.04 1.81 0.9107 8.7 ± 0.3 8.1 0.2691
Human, donor 2 (n = 12) 3 days 1.52 ± 0.15 1.29 1.91 ± 0.09 1.75 0.4783 9.0 ± 0.4 8.6 0.7983
Human, donor 3 (n = 12) 3 days 0.98 ± 0.28 0.56 1.88 ± 0.11 1.77 0.9701 9.0 ± 0.5 8.4 0.8112
Rhesus (n = 5) w/o 2.02 ± 0.37 1.58 1.91 ± 0.06 1.87   8.2 ± 0.5 7.4  
Marmoset (n = 5) w/o 1.37 ± 0.16 1.16 1.87 ± 0.07 1.79   8.2 ± 0.3 7.8  
Rat (n = 5) w/o 3.80 ± 0.78 2.48 1.95 ± 0.04 1.91   8.3 ± 1.0 6.8  
Mouse (n = 5) w/o 5.67 ± 0.61 4.65 1.95 ± 0.01 1.93   8.2 ± 0.5 7.3  
  1. The average is indicated ± STABW. Student’s t-test was performed to compare absorbance 260/280 and RIN values with and without an incubation period, p-values are indicated
  2. n indicates the number of analysed samples, w/o without