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Table 3 cRNA yield after sample preparation with 3′ IVT PLUS Reagent Kit (Affymetrix)

From: RNA isolation from precision-cut lung slices (PCLS) from different species

Material

Absorbance 260/230

Yield cRNA (µg)

A549 cells

1.90 ± 0.17

58.6 ± 15.6

Human PCLS

0.60 ± 0.21

51.5 ± 12.1

  1. 250 ng RNA were used as starting material. Results represent the means of 20 samples, all samples with A260/A280 ratios around 1.9 and RIN values above 8.0
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BMC Research Notes

ISSN: 1756-0500

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