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Table 3 cRNA yield after sample preparation with 3′ IVT PLUS Reagent Kit (Affymetrix)

From: RNA isolation from precision-cut lung slices (PCLS) from different species

Material Absorbance 260/230 Yield cRNA (µg)
A549 cells 1.90 ± 0.17 58.6 ± 15.6
Human PCLS 0.60 ± 0.21 51.5 ± 12.1
  1. 250 ng RNA were used as starting material. Results represent the means of 20 samples, all samples with A260/A280 ratios around 1.9 and RIN values above 8.0