Larvicidal activity of phytosteroid compounds from leaf extract of Solanum nigrum against Culex vishnui group and Anopheles subpictus

Table 2 Probit and regression analyses of mortality rates of early 3rd instar larvae of Culex vishnui group and Anopheles subpictus to different concentrations of bioactive compound isolated from mature leaves of Solanum nigrum

Mosquito type	Time of exposure (h)	LC₅₀ (mg/L)	LC₅₀ (mg/L) LCL–UCL	LC₉₀ (mg/L)	Regression equation	R value
Cx. Vishnui group	24	14.48	0.28–23.79	121.09	Y = 0.12x + 12.75	0.77
	48	15.89	5.37–22.48	61.40	Y = 0.17x + 12.62	0.90
	72	5.64	0.00–14.68	44.33	Y = 0.08x + 18.55	0.63
An. subpictus	24	13.21	1.15–21.36	76.27	Y = 0.13x + 13.81	0.79
	48	9.93	0.25–53.38	41.81	Y = 0.06x + 19.52	0.51
	72	3.68	0.56–16.46	24.74	Y = 0.26x + 84.30	0.98

LC lethal concentration, LCL lower confidence limit, UCL upper confidence limit, R regression coefficient value

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