Fig. 2
a The PCR sensitivity levels and showing the 166 bp amplicon using a tenfold serial dilution of E. histolytica DNA. b The sensitivity of the stem 18S LAMP test with outer primers. The LAMP amplicons were less bright with the forma without outer primers F3/B3 (image not shown) but the sensitivity levels were identical. c The visual appearance of stem 18S LAMP test amplification product after addition of 1/10 dilution of SYBR® Green I dye. The dye fluoresces strongly when bound to the double stranded DNA and the resulting DNA-dye-complex gives a green colour while fluorescence is minimal when the dye is free in the solution and gives orange/brown colour. Samples 1, 2, 3 and 5 are E. histolytica positive and samples 4 and 6 are negative samples. C control, N negative control