Stem loop-mediated isothermal amplification test: comparative analysis with classical LAMP and PCR in detection of Entamoeba histolytica in Kenya

Table 2 The analytical sensitivity of LAMP tests based on 18S rRNA gene and PCR using a tenfold serial dilution of E. histolytica DNA

Test	Combination	Tenfold serial dilution						C_T value	Remarks
Test	Combination	Neat	10^{−1 to 4}	10⁻⁵	10⁻⁶	10⁻⁷	10⁻⁸	C_T value	Remarks
Stem LAMP^a	F3/B3, FIP/BIP, LF/LB, SF/SB	+	+	+	+	+	−	28	This study
Stem LAMP^b	FIP/BIP, LF/LB, SF/SB	+	+	+	+	+	−	28	This study
Standard LAMP^c	F3/B3, FIP/BIP, LF/LB	+	+	+	+	−	−	33	This study
Standard LAMP^d	F3/B3, FIP/BIP	+	+	±	−	−	−	–	This study
Published LAMP^e	F3/B3, FIP/BIP	+	+	+	−	−	−	39	Liang et al. [32]
PCR test	EntaF and EhR	+	+	+	−	−	−	nd	Hamzah et al. [20]

The cycle threshold (C_T) in minutes for tenfold dilution of 10⁻⁵ (3 ng/ml)
Neat = ~300 µg/µl
nd not done
^aLAMP test with outer F3/B3 primers
^bLAMP test without outer F3/B3 primers (amplicons are less bright)
^cStandard LAMP test (the most common LAMP format)
^dStandard LAMP test format without loop primers (initial format, not commonly used)
^ePublished LAMP test without loop primers
± Half of the replicates were positive (2 out of 4)
The lower the value the greater the amount of target DNA formed (in italics)

ISSN: 1756-0500