Table 1 Purification yield of rhGM-CSF from P. pastoris and S. cerevisiae using IEC with % elution buffer or HIC
Sample | GM-CSF from P. pastoris (IEC) 10% | GM-CSF from P. pastoris (IEC) 20% | GM-CSF from P. pastoris (HIC) | GM-CSF from S. cerevisiae (IEC) 10% | GM-CSF from S. cerevisiae (IEC) 20% | GM-CSF from S. cerevisiae (HIC) |
|---|---|---|---|---|---|---|
Purity (%) | 92.7 | 95.2 | 85.2 | 95.6 | 64.22 | 42.9 |
Purification yield (%) | 56.9 | 35.9 | 53.8 | 21.0 | 30.8 | 0.5 |