The conditional expression of Pdxk in the Drosophila eye results in reduced ommatidia number and increased disruption of the eyes and is suppressed upon co-expression with Buffy.
a Scanning electron micrographs when Pdxk is inhibited in the eye (I) GMR-Gal4/UAS-lacZ and (II) GMR-Gal4/Pdxk-RNAi. b The biometric analysis of the eyes indicated a slight decrease in mean ommatidia number and a higher percent disruption of the eye when compared to the control. c The co-expression of Pdxk-RNAi with α-synuclein-expression (I) UAS-α-synuclein; GMR-Gal4/UAS-lacZ and (II) UAS-α-synuclein; GMR-Gal4/Pdxk-RNAi. Biometric analysis of α-synuclein-expression and Pdxk inhibition in the developing eye revealed no significance in the number of ommatidia and the degree of ommatidial disruption. d Co-expression of Buffy with Pdxk-RNAi (I) UAS-Buffy; GMR-Gal4/UAS-lacZ and (II) UAS-Buffy; GMR-Gal4/Pdxk-RNAi. Biometric analysis showed restoration of the mean number of ommatidia and ommatidial disruption to control levels. Comparisons were determined by unpaired two-tailed T test (P < 0.05), error bars are SEM, n = 10 and asterisks represent statistical significance