Table 2 Details for the 15 selected, validated Sium latifolium microsatellite loci
Locus | Sequence identifier and accession no. | Primer sequences (5′–3′) | Repeat motif | T (°C) |
|---|---|---|---|---|
Sla01 | GWP00014, LN849725 | F: [6FAM]AGACTTGTATGTCCTGCATTATGTTC R: CAGCTGGTGAAGCCAATTTAG | (GT)13 | 58 |
Sla02 | GWP00025, LN849726 | F: [HEX]TTGCCTCAAGTGCAGAACAG R: CAACCACTTACATATGTTCACAATACC | (CT)15 | 58 |
Sla03 | GWP00030, LN849727 | F: [6FAM]ACCAATGACAAGTGGGTTCC R: CCCAAGATTTCCTTGAAGTACAG | (CA)28 | 53 |
Sla04 | GWP00089, LN849728 | F: [HEX]GATTCCCGATCTCCAATTCC R: CGCGACATCGAAGAGTTTG | (CA)13 | 53 |
Sla05 | GWP00130, LN849729 | F: [6FAM]AGAAGCACGCTATTGCACTG R: CATTTGTCAGTTGTCACATACCC | (GT)10 | 58 |
Sla06 | GWP00133, LN849730 | F: [6FAM]TTGCAAGGAAACTGAGACCAC R: TGGACATTGTACCAGCTACCC | (CT)14 | 51 |
Sla07 | GWP00178, LN849731 | F: [6FAM]GGACATCTAAGCATAAAGTGCAATAAC R: TTGTTTCTAGCAGAGGTAGCTTGAC | (CA)18 | 58 |
Sla08 | GWP00226, LN849732 | F: [HEX]CAGATGGATAGTTGAAACCAAGTG R: TTAAGTTAGACAAGCGGCCTTC | (CA)12 | 51 |
Sla09 | GWP00268, LN849733 | F: [HEX]CAGCAAGAATTGCCAATCG R: AATGGTGAAGGGAAATGCTG | (GT)12 | 58 |
Sla10 | GWP00318,LN849734 | F: [HEX]TTACTTGCCCACGCTTCTG R: TCTTCTAAAGCAGGGGAGTACG | (CT)15 | 51 |
Sla11 | GWP00319, LN849735 | F: [6FAM]TGATACGGTGGATGATGAGC R: TGCATTATATGCGTCAACTGG | (GT)12 (GA)8 | 58 |
Sla12 | GWP00373, LN849736 | F: [6FAM]GCCACAGTAGATCCATTACTCAAC R: TTTGACACAGATTGGAATCCTC | (GT)16 | 51 |
Sla13 | GWP00423, LN849737 | F: [HEX]CCTTAACTAAAGACTAAAGACTGTGGAAC R: ACTTGGTCGGTTATGTTGTGG | (GA)13 | 58 |
Sla14 | GWP03443, LN849738 | F: [6FAM]CTGGCAAACACACGCAAC R: TTTCTTTGTTTGGGTTTGATCTC | (GA)13 | 58 |
Sla15 | GWP03601, LN849739 | F: [6FAM]TTGTAAACGCCCTTACCATTG R: AATAAACCATGAACAGATGAAGATTG | (GT)15 | 51 |