The characterisation of microsatellite markers reveals tetraploidy in the Greater Water Parsnip, Sium latifolium (Apiaceae)

Table 3 Characterisation of 15 dinucleotide microsatellite loci for the Greater Water Parsnip Sium latifolium, all tested on 24 individuals sampled at Wickhampton Marshes, reveals tetraploidy in this species

Locus	Fluoro dye	Exp. I50 (bp),	Obs. I50 (bp).	N	K	Observed allele size range (bp)	Number of individuals with 1–2 alleles	Number of individuals with 3–4 alleles	Ho
Sla01	[6FAM]	192	191, 193, 195	23	12	189–213	0	23	1.000
Sla02	[HEX]	154	132, 150, 154, 164	24	17	132–180	4	20	1.000
Sla03	[6FAM]	241	230, 232, 240	23	16	202–242	10	13	1.000
Sla04	[HEX]	196	180, 188, 194*	24	9	180–204	9	15	1.000
Sla05	[6FAM]	248	244, 248, 250	23	8	242–254	8	15	0.958
Sla06	[6FAM]	154	130, 138, 150, 154	23	9	130–158	5	18	0.958
Sla07	[6FAM]	228	203, 207, 224*	24	12	203–224	2	22	1.000
Sla08	[HEX]	115	104, 110, 112*	24	15	94–136	3	21	1.000
Sla09	[HEX]	180	168, 182*	24	10	166–186	21	3	0.875
Sla10	[HEX]	142	132, 141	23	13	128–170	14	9	1.000
Sla11	[6FAM]	148	136, 142, 148	22	11	128–156	8	14	1.000
Sla12	[6FAM]	107	92, 106, 108, 112	22	13	90–116	3	19	1.000
Sla13	[HEX]	121	117, 128*	23	11	110–136	11	12	1.000
Sla14	[6FAM]	161	158, 160, 168	22	14	134–176	4	18	1.000
Sla15	[6FAM]	106	101, 105, 107, 119	23	12	83–119	7	19	1.000

Microsatellite loci, expected and observed allele sizes (with the sequenced allele underlined*; bp) of individual from which the microsatellite sequences were isolated (individual I50, sampled at Wickhampton Marshes, Norfolk), number of individuals successfully genotyped (n), number of alleles (k), allele size range (bp), observed heterozygosity (Ho). Exp. I50 (bp), Expected allele size of I50, Obs. I50 (bp), Observed amplified allele sizes of individual, I50, *Minor size differences (bp) were observed between the expected size of the allele (based on sequencing) and observed allele size (based on ABI genotyping). This error is caused by (1) the presence of the fluorescent dye label (6FAM and HEX) and/or (2) sequence misalignment due to the repeat region when creating the consensus sequence from the two paired-end complementary sequences

ISSN: 1756-0500