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Table 3 Characterisation of 15 dinucleotide microsatellite loci for the Greater Water Parsnip Sium latifolium, all tested on 24 individuals sampled at Wickhampton Marshes, reveals tetraploidy in this species

From: The characterisation of microsatellite markers reveals tetraploidy in the Greater Water Parsnip, Sium latifolium (Apiaceae)

Locus Fluoro dye Exp. I50 (bp), Obs. I50 (bp). N K Observed allele size range (bp) Number of individuals with 1–2 alleles Number of individuals with 3–4 alleles Ho
Sla01 [6FAM] 192 191, 193, 195 23 12 189–213 0 23 1.000
Sla02 [HEX] 154 132, 150, 154, 164 24 17 132–180 4 20 1.000
Sla03 [6FAM] 241 230, 232, 240 23 16 202–242 10 13 1.000
Sla04 [HEX] 196 180, 188, 194* 24 9 180–204 9 15 1.000
Sla05 [6FAM] 248 244, 248, 250 23 8 242–254 8 15 0.958
Sla06 [6FAM] 154 130, 138, 150, 154 23 9 130–158 5 18 0.958
Sla07 [6FAM] 228 203, 207, 224* 24 12 203–224 2 22 1.000
Sla08 [HEX] 115 104, 110, 112* 24 15 94–136 3 21 1.000
Sla09 [HEX] 180 168, 182* 24 10 166–186 21 3 0.875
Sla10 [HEX] 142 132, 141 23 13 128–170 14 9 1.000
Sla11 [6FAM] 148 136, 142, 148 22 11 128–156 8 14 1.000
Sla12 [6FAM] 107 92, 106, 108, 112 22 13 90–116 3 19 1.000
Sla13 [HEX] 121 117, 128* 23 11 110–136 11 12 1.000
Sla14 [6FAM] 161 158, 160, 168 22 14 134–176 4 18 1.000
Sla15 [6FAM] 106 101, 105, 107, 119 23 12 83–119 7 19 1.000
  1. Microsatellite loci, expected and observed allele sizes (with the sequenced allele underlined*; bp) of individual from which the microsatellite sequences were isolated (individual I50, sampled at Wickhampton Marshes, Norfolk), number of individuals successfully genotyped (n), number of alleles (k), allele size range (bp), observed heterozygosity (Ho). Exp. I50 (bp), Expected allele size of I50, Obs. I50 (bp), Observed amplified allele sizes of individual, I50, *Minor size differences (bp) were observed between the expected size of the allele (based on sequencing) and observed allele size (based on ABI genotyping). This error is caused by (1) the presence of the fluorescent dye label (6FAM and HEX) and/or (2) sequence misalignment due to the repeat region when creating the consensus sequence from the two paired-end complementary sequences