Fig. 2From: Growth-dependent activity of the cold shock cspA promoter + 5′ UTR and production of the protein CspA in Staphylococcus aureus NewmanA chromosomal arrangement of regulatory sequences and two proposed cspA transcriptional start points in Staphylococcus aureus. The location and length in base pairs (bp) for the promoter + 3′ sequence (arrow) and coding sequence (cds) (rectangle) for msaA in light blue are shown in relation to the length and location of the promoter + 5′ UTR (arrow) and cds (rectangle) for cspA in red. The TSP for cspA identified by primer extension is identified by a green bent arrow and located by a 112 bp dark blue overline upstream of the 200 bp coding sequence for the cspA gene in red. The initial promoter for cspA/msaB identified by Sahukhal and Elasri [20] is identified by a red bent arrow at the 5′ end of the proposed promoter for msaA in blue and located by a 514 bp red overline upstream of the cds for cspA [20]. The red 192 bp promoter + 5′ UTR of cspA designed for the XylE assays is labeled. The location of the 324 bp regulatory sequence used for XylE assays is marked by a green overline [20]. The relative locations of these features are based on sequences from S. aureus Newman (NC_009641.1) [33]Back to article page