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Table 1 Bacterial strains and plasmids

From: Growth-dependent activity of the cold shock cspA promoter + 5′ UTR and production of the protein CspA in Staphylococcus aureus Newman

Escherichia coli
 DH5α F Φ80 lacZ ΔM15Δ(lacZA-argF) U169 deoR recA1 endA1 hsdR17 (r K m K) phoA supE44 λ thi-1 gyrA96 relA1 Invitrogen™
 BL21(DE3)pLysS F ompT hsdS B (r B ,m B ) gal dcm (DE3) pLysS (CamR) Invitrogen™
 SKEPrtEx E. coli BL21(DE3)pLysS with plasmid pSKPrtExA This study
Staphylococcus aureus
 RN4220 res strain [23]
 SKC31 A Km cassette deletion–insertion of the cspA coding region in S. aureus COL, TetR [10]
 Newman Clinical isolate, strong positive coagulase [21]
 SKN23 A Km cassette deletion–insertion of the cspA coding region in S. aureus Newman This study
Plasmids
 pCR2.1 TA cloning vector Invitrogen™
 pSKCPriEx pCR2.1 with 238 bp insert of cspA coding region, promoter + 5′ UTR This study
 pLL38 E. coli-staphylococcal shuttle vector that contains promoterless xylE as a reporter gene for promoter-gene fusion; gram-positive RBS (GGAGG) [29]
 pSK51 pLL38 containing a putative sequence of −35 and −10 sites plus P3, −53 bp putative TSP of cspA, ligated to a xylE reporter gene This study
 pSK88 pLL38 containing a putative sequence of −5 and −10 sites plus P2, −88 bp putative TSP of cspA ligated, to a xylE reporter gene This study
 pSK96 pLL38 containing a putative 96 bp sequence of the cspA promoter: upstream protein binding sites, −35 and −10 sites ending at P1, −112 bp putative TSP of cspA ligated to a xylE reporter gene This study
 pSK192 pLL38 containing the sequence of pSK96 + 5′ UTR of cspA This study
 pSK324 pLL38 containing msaB promoter insert from pMOE 482; 5′ PstI and 3′ EcoRI sites [20]; This study
 pRHB152 pRHB153 with six His-tag replacement of GST-tag for protein overexpression [25]
 pSKPrtExpA pRHB152 with six His-tag 200 bp coding region for CspA overexpression This study