Table 1 The kits tested plus the modified CTAB method are listed along with a summary of results
From: A practical examination of RNA isolation methods for European pear (Pyrus communis)
Method | Tissue | |||||||
|---|---|---|---|---|---|---|---|---|
d’Anjou peel stored | d’Anjou cortex stored | d’Anjou peel fresh | d’Anjou cortex fresh | Bartlett peel fresh | Bartlett cortex fresh | Gala peel fresh | Gala cortex fresh | |
Macherey–Nagel NucleoSpin RNA Plant buffer RAI Cat # 740949 | X | X | – | – | – | – | – | – |
Macherey–Nagel NucleoSpin RNA Plant buffer RAP Cat # 740949 | √ a | OKc | √ e | √ g | √ | √ | √ | √ |
Omega E.Z.N.A. HP Total RNA Cat # R6812-00 | √ | X | – | – | – | – | – | – |
Omega E.Z.N.A. Total RNA Cat # R6834-00 | √ | X | – | – | – | – | – | – |
Omega E.Z.N.A. Plant RNA Cat # R6827-00 | X | Xnr | – | – | – | – | – | – |
ThermoFisher GeneJET Plant RNA Cat # K0809 | OK | X | – | – | – | – | – | – |
Qiagen RNeasy Plus Universal Cat # 73404 | X | Poor | – | – | √ | √ | – | – |
Qiagen RNeasy Plant buffer RLC Cat # 74903 | √ | Poor | – | – | √* | √* | – | – |
Qiagen RNeasy Plant buffer RLT Cat # 74903 | OK | X | – | – | – | – | – | – |
Zymo ZR Plant RNA Cat # R2024 | X | X | – | – | – | – | – | – |
CTAB modified from Gapper et al. [18] | √ b | √d | √f | √ h | √ | √ | √ | √ |