Simple and fast quantification of DNA damage by real-time PCR, and its application to nuclear and mitochondrial DNA from multiple tissues of aging zebrafish

Table 1 Primers and targets

Primer	Sequence	Amplicon (bp)	Target
AHRLF	GTCCTTGCAGGTTGGCAAATGG	3502	AHR2
AHRLR	GACCTTGTCTGGTTTTCATCCC
AHRSLF	TCATCCTGTTATCCACCACACTGTTG	1637	AHR2
AHRSLR	TGGTTCTTGGCTACACTGAGATTGAG
AHRRF	CCAAGGTCCGACATAACTCACTTCTG	55	AHR2
AHRRR	GACATGATGTACTGTGCTGACAACCA
mtLF	TTAAAGCCCCGAATCCAGGTGAGC	3669	mtDNA coding region
mtLR	TTAGGGGTAGTGAGTTTTGGGTC
mtSLF	GGATTCCAAGACGCAGCATCACCTG	1978	mtDNA coding region
mtSLR	GGAGCGGCACTTCAAATGGGTCAAG
mtDLPF	CCTTACACGATTCTTCGCATTCCAC	1939	mtDNA D-loop region
mtDLPR	GGCTTGGCTAGGCGTCTTGG
mtRF	CGAGGAGCAGGTATCAGGCACA	55	mtDNA coding region
mtRR	GTGGCTTGGCTAGGCGTCTTG

ISSN: 1756-0500