Fig. 1

Phylogenetic tree including the distribution of important virulence genes and loci. The maximum likelihood approximated tree based on 50,643 SNPs, shows that 30 isolates from healthy and diseased chickens (C) and the turkeys (T) did not cluster into representative groups. NELoc-1, -3 and cnaA were significantly present in NE isolates from chickens, whereas NELoc-2 was associated with NE isolates from both chickens and turkeys. Reference strain C perfringens ATCC 13124 is not included. The scale represents substitutions per site