Table 1 List of PCR primers
Set | Targets | Strand | Name of primer | Start | End | Sequence (5′-3′) | Size of PCR product |
|---|---|---|---|---|---|---|---|
E1 | miR-BARTs3,4,1 | Forward | EBV139081F | 139,081 | 139,100 | tccctgtaaacacacaccac | 440 |
Reverse | EBV139520R | 139,520 | 139,501 | ttctacatcatgcctggttc | |||
E2 | miR-BARTs15,5,16,17,6 | Forward | EBV139501F | 139,501 | 139,520 | gaaccaggcatgatgtagaa | 690 |
Reverse | EBV140190R | 140,190 | 140,171 | tttagatctgtggttacatg | |||
E3 | miR-BARTs21,18 | Forward | EBV145451F | 145,451 | 145,470 | ttagatgttagctttgtgtt | 590 |
Reverse | EBV146040R | 146,040 | 146,021 | ggcccaaaccttcgcagcag | |||
E4 | miR-BART7 | Forward | EBV145911F | 145,911 | 145,930 | ttgttgccgttgaaagacgg | 610 |
Reverse | EBV146520R | 146,520 | 146,501 | tggccacactaaacacacaa | |||
E5 | miR-BARTs8,9 | Forward | EBV146701F | 146,701 | 146,720 | ttatttgggttacaagacct | 350 |
Reverse | EBV147050R | 147,050 | 147,031 | cacaatgaaacccaaagccc | |||
E6 | miR-BARTs22, 10,11 | Forward | EBV147131F | 147,131 | 147,150 | cggttgtcacaggtgctaga | 500 |
Reverse | EBV147630R | 147,630 | 147,611 | cgtgaaaggcactccagaat | |||
E7 | miR-BARTs12,19,20 | Forward | EBV147871F | 147,871 | 147,890 | acctaagacccgcccatcac | 550 |
Reverse | EBV148420R | 148,420 | 148,401 | ccaaaggacccgggatcacg | |||
E8 | miR-BARTs13, 14 | Forward | EBV148461F | 148,461 | 148,480 | catcttgacgttggaatgtc | 360 |
Reverse | EBV148820R | 148,820 | 148,801 | ctcctgggttggcgtttccg | |||
E9 | miR-BART2 | Forward | EBV152651F | 152,651 | 152,670 | gcagcaaaagaggaacttgc | 350 |
Reverse | EBV153000R | 153,000 | 152,981 | ggcaaagatccccagcggag | |||
E10 | miR-BHRF1-1 | Forward | EBV41581F | 41,581 | 41,600 | cctcaccatgacacactaag | 260 |
Reverse | EBV41840R | 41,840 | 41,821 | ccagatgcacccaacagccc | |||
E11 | miR-BHRF1-2,3 | Forward | EBV42991F | 42,991 | 43,010 | gggtgacacagtgcccatgc | 330 |
Reverse | EBV43320R | 43,320 | 43,301 | acactcacctcagttatttc |