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Table 1 Primer sequences and descriptions of 22 microsatellite markers developed in Acer saccharum

From: Development of novel genic microsatellite markers from transcriptome sequencing in sugar maple (Acer saccharum Marsh.)

Locus

Primer sequences (5′–3′)

Repeat EdEdmotif

Size range (bp)

**Expected fragment size

Na

Ho

He

F

p

As_di1

F: TCCCAGGCATGAACAAGGTT

R: TGCAGTAAGTTGACAGCTCT

(AC)9

251–273

230

9

0.581

0.658

0.117

0.0034

As_di7

F: GGGTCTGTCTCTGTTTCTGCA

R: ACAGGGTTCACTGAGCTGTG

(AC)9

151–161

134

6

0.651

0.602

0.081

0.2578

As_di9a

F: TGCTGGAAAGTGGAACCTGT

R: AGTCTGATCTGTCATGGGCTC

(TA)8

121–147

100

12

0.375

0.835

0.551

<0.0001**

mAs_di11

F: AGAGAACCACCAAGGATGCA

R: CAGGGAGCCATTTCACTCTGA

(GA)8

187

167

1

0

0

–

–

As_di12

F: AAGACATCTTGAGGGCGGTG

R: TGTAACTGCATAACGGGCCA

(TC)9

447–458

425

4

0.194

0.271

0.283

0.0014*

mAs_di13

F: TCAAGAAATACTGGCTCAGGTCA

R: ACATGCATGTTGAGCGATTGT

(TC)8

264

238

1

0

0

–

–

As_di15

F: GGGCAGAGAGGGAATTCGAG

R: TGGGGAGACAGAACTTGTGC

(TC)8

259–265

235

4

0.375

0.429

0.126

0.0236

As_di16

F: AATTGCCTGTGGTGGGAACT

R: ACTCCACTCTCTTTCTTGCTCA

(GT)8

211–218

190

4

0.675

0.680

0.007

0.8696

mAs_di19

F: GACCTGACCACCTCCTCCTA

R: AGGGCAACATACACGGATCG

(CT)9

228

205

1

0

0

–

–

As_di21

F: TGTCAGCAGCCCTACAGTTG

R: ACAGGTCACGATCTCTCCCT

(GT)8

135–142

113

4

0.450

0.609

0.261

0.0455

mAs_di27

F: TCATGACCATGACCCAACACT

R: TCCTGTGGATTCTTTTGTATCTGGT

(TC)8

386

363

1

0

0

–

–

mAs_di30

F: GATCCCCTTCGTTGCTGACA

R: GGACTGCCCGATTGATACTCA

(TG)9

457

432

1

0

0

–

–

mAs_di31

F: CTCCACCACCATCCAACCAA

R: TCCTCAGCTTCTTGGGCTTG

(AC)8

187–189

167

1

0

0

–

–

As_di34a

F: AACGGATGGCAAGCTAGCTT

R: CAGGCCTGCCCAGAAACTAA

(AC)8

225–240

218

4

0.217

0.726

0.701

<0.0001**

As_di35

F: TGTTAGTCTCCTCCACACGT

R: CAGCAGCAGCAGCAAAACT

(TC)8

151–153

129

2

0.143

0.224

0.362

0.0751

As_di36

F: ATGTGAGTCCGTGAGTCCGT

R: ATAAGCAGCAGCAGCAGACA

(TG)8

237–245

212

5

0.475

0.606

0.216

0.0322

As_di37

F: TGGTGGGTAGCAGCAAAAGA

R:TGCACATGGGATGATGATCAGT

(TG)11

167–184

151

8

0.722

0.704

−0.025

0.9324

As_di38

F: ACAGAGAGAGAGAGAGCTTGT

R: TACCGTCATGGCCGATGATG

(AC)8

175–179

154

3

0.184

0.362

0.491

0.0012*

As_di41

F: AAGCTGAGAAACCCAAAGCA

R: CACCACCCAACCCTTTTCCT

(TA)8

259–271

236

6

0.526

0.625

0.157

0.2675

As_di48

F: AGGTTCGGGTTTTGAATCTTCA

R: CAAGGACTTTGGCTCTGCTG

(TA)8

173–179

155

4

0.200

0.678

0.705

<0.0001**

As_di49

F: TGCAACTGTTGAGTGGTGGA

R: ACAAGTCGAACAACCCGTTG

(TG)10

159–183

133

10

0.625

0.636

0.017

0.0528

As_tetra1

F: TTGACGGAGAGCTTGGTTCC

R: AAAACCCAATTCGCCACGTG

(TGCT)6

257–273

241

5

0.341

0.337

−0.012

0.4674

+Mean

    

6

0.424

0.543

0.222

 

Mean

    

4

0.308

0.395

0.222

 
  1. N a number of alleles per locus, H o observed heterozygosity, H e expected heterozygosity, F inbreeding coefficient
  2. * Significantly different from Hardy–Weinberg proportions (α = 0.05) after Bonferroni corrections
  3. ** Significantly different from Hardy–Weinberg proportions (α = 0.01) after Bonferroni corrections m: these markers amplified monomorphic loci and were not tested on the whole population. **: expected fragment size based on EST contigs. Actual fragments were longer as tailed primers were used for amplification. +Mean variation was calculated for polymorphic markers only to allow for comparisons with other related studies on sugar maple [4]
  4. a Markers As_di34 and As_di9 did not amplify in nearly half of the samples and should not be used for population level analyses
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BMC Research Notes

ISSN: 1756-0500

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