Skip to main content
Fig. 1 | BMC Research Notes

Fig. 1

From: A quantitative and qualitative comparison of illumina MiSeq and 454 amplicon sequencing for genotyping the highly polymorphic major histocompatibility complex (MHC) in a non-model species

Fig. 1

Mean proportion of reads of the first 50 variants (true and artefact alleles) in amplicons with different numbers of putative true alleles (A i ) for the MiSeq (a) and the 454 (b) data. The mean sequencing depth for each allelic level (i.e. putative alleles ordered by depth) was calculated as the total number of reads from all successfully genotyped amplicons per allelic level divided by the total reads per amplicon. These calculations were performed separately on amplicons grouped by the number of putative alleles they possessed (A i  = 3 to 9 alleles). Total numbers of amplicons: N MiSeq = 76, N 454 = 68. Grey bars show the sequencing depths of true alleles, whereas black bars show the sequencing depths of artefacts

Back to article page