Fig. 9From: Design and implementation of a custom next generation sequencing panel for selected vitamin D associated genesNGS and Sanger sequencing results at chromosome 20, location 52789779. The Partek figure on the left shows a C/A variant in sample RT106 identified in pool 2 of the Ion PGM™ sequencing results. This was not called by the Ion S5™ XL. Sanger sequencing on the right confirmed that there is no variant at this locus in either sample RT106 or sample LT744. A single peak is shown for C in both of these samples (the Sanger sequencing image to the right of this image is shown in the reverse complement). These results confirm that the Ion PGM™ called this variant incorrectlyBack to article page