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Table 3 Sequencing runs completed using both the Ion PGM™ and Ion S5™ XL

From: Design and implementation of a custom next generation sequencing panel for selected vitamin D associated genes

Sequencing Runs per chip

Sequencer

Chip type

Dilution (pM)

Preparation

Max. output

Samples

Usable reads (%)

Max. reads

Polyclonal (%)

Ion PGMâ„¢

Ion 316â„¢

26

Ion Chefâ„¢

429 Mb

8–12

51–57

2,755,634

21–37

Ion PGMâ„¢

Ion 318â„¢

26/50

Ion Chefâ„¢/Manual

668 Mb

48–96

48–50

4,880,500

11–40

Ion S5â„¢ XL

Ion 530â„¢

50

Ion Chefâ„¢

3.22 Gb

85

50–61

80,308,654

26–30

  1. Libraries were prepared using 20 ng genomic DNA. Dilution factors chosen for the sequencing runs were either 26 or 50 pM depending on the number of samples to be sequenced and the level of coverage desired
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BMC Research Notes

ISSN: 1756-0500

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