Table 1 Reference standard positive ATCC specimens used in the validation of the new primer set, and species identification of positive clinical specimens
From: Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae
Source | Specimen | Species | Reference standard assay positivea |
|---|---|---|---|
ATCC | ATCC50372 | A. polyphaga | Both |
ATCC30173 | A. polyphaga | Both | |
ATCC50492 | A. polyphaga | Both | |
ATCC PRA-220 | Acanthamoeba spp. | Both | |
ATCC50372 | A. polyphaga | Both | |
ATCC50373 | A. castellanii | Both | |
ATCC50492 | A. castellanii | Both | |
ATCC50493 | A. castellanii | Both | |
ATCC50739 | A. castellanii | Both | |
ATCC30171 | A. culbertsoni | Qvarnstrom | |
Clinical specimen | 1 | A. castellanii | Both |
2 | A. hatchetti | Qvarnstrom | |
3 | A. castellanii | Both | |
4 | A. castellanii | Qvarnstrom | |
5 | Unknown | Qvarnstrom | |
6 | A. polyphaga | Both | |
7 | Unknown | Riviere | |
8 | A. lenticulata | Riviere | |
9 | A. castellani | Riviere | |
10 | A. griffini | Riviere | |
11 | Unknown | Riviere | |
12 | A. castellanii | Both | |
13 | A. castellanii | Both | |
14 | Unknown | Riviere | |
15 | A. lenticulata | Qvarnstrom | |
16 | Unknown | Riviere | |
17 | A. hatchetti | Both | |
18 | A. polyphaga | Both | |
19 | Unknown | Qvarnstrom | |
20 | Unknown | Qvarnstrom | |
21 | A. quina | Qvarnstrom |