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Table 3 Performance characteristics of the new assay compared to the Qvarnstrom assay [7]

From: Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae

Primer Set

Positive (N)

Negative (N)

Sensitivity (%)

Specificity (%)

PPV (%)

NPV (%)

New primer set

29

78

100% (95% CI 82.8–100%)

94.0% (95% CI 85.9–97.8%)

82.8

100

  1. By Qvarnstrom, 24 specimens were considered true positive, and 83 were considered true negative