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Table 3 Performance characteristics of the new assay compared to the Qvarnstrom assay [7]

From: Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae

Primer Set Positive (N) Negative (N) Sensitivity (%) Specificity (%) PPV (%) NPV (%)
New primer set 29 78 100% (95% CI 82.8–100%) 94.0% (95% CI 85.9–97.8%) 82.8 100
  1. By Qvarnstrom, 24 specimens were considered true positive, and 83 were considered true negative