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Table 4 Performance characteristics of the new assay compared to the composite reference standard of either or both Qvarnstrom [7] and Riviere [6] assay positivity

From: Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae

Primer Set Positive (N) Negative (N) Sensitivity (%) Specificity (%) PPV (%) NPV (%)
New primer set 29 78 93.5% (95% CI 77.2–98.9%) 100% (95% CI 94.0–100%) 100 97.4
  1. By either or both of Qvarnstrom and Riviere, 31 specimens were considered true positive, and 76 were considered true negative