From: Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae
Primer Set | Positive (N) | Negative (N) | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) |
---|---|---|---|---|---|---|
New primer set | 13 | 24 | 100% (95% CI 69.9–100%) | 96.0% (95% CI 77.7–99.8%) | 92.3 | 100 |
Riviere assay [6] | 11 | 26 | 91.7% (95% CI 59.8–99.6%) | 100% (95% CI 83.4–100%) | 100 | 96.1 |
Qvarnstrom assay [7] | 12 | 25 | 100% (95% CI 69.9–100%) | 100% (95% CI 83.4–100%) | 100 | 100 |