Skip to main content

Table 5 Performance characteristics of the new assay, as well as Riviere [6] and Qvarnstrom [7] compared to culture as the reference standard

From: Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae

Primer Set

Positive (N)

Negative (N)

Sensitivity (%)

Specificity (%)

PPV (%)

NPV (%)

New primer set

13

24

100% (95% CI 69.9–100%)

96.0% (95% CI 77.7–99.8%)

92.3

100

Riviere assay [6]

11

26

91.7% (95% CI 59.8–99.6%)

100% (95% CI 83.4–100%)

100

96.1

Qvarnstrom assay [7]

12

25

100% (95% CI 69.9–100%)

100% (95% CI 83.4–100%)

100

100

  1. By culture, 12 specimens were considered true positive and 25 specimens were considered true negative