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Table 5 Performance characteristics of the new assay, as well as Riviere [6] and Qvarnstrom [7] compared to culture as the reference standard

From: Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae

Primer Set Positive (N) Negative (N) Sensitivity (%) Specificity (%) PPV (%) NPV (%)
New primer set 13 24 100% (95% CI 69.9–100%) 96.0% (95% CI 77.7–99.8%) 92.3 100
Riviere assay [6] 11 26 91.7% (95% CI 59.8–99.6%) 100% (95% CI 83.4–100%) 100 96.1
Qvarnstrom assay [7] 12 25 100% (95% CI 69.9–100%) 100% (95% CI 83.4–100%) 100 100
  1. By culture, 12 specimens were considered true positive and 25 specimens were considered true negative