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Fig. 3 | BMC Research Notes

Fig. 3

From: Authentication of collagen VI antibodies

Fig. 3

Detection of the α2 chain. Immunostaining was conducted on cross- (A, D, H, K, O, S and W) and transverse (B, E, I, L, P, T and X) sections of skeletal muscle. Blots containing reduced and non-reduced sets of lysates from cells expressing α1, α2 and α3 chains individually (C, F, J, M, Q, U and Y) were probed with each α2 antibody. Blots containing multimeric collagen VI expressing all three chains and electrophoresed under non-reducing conditions were probed (G, N, R, V and Z). Migration position of molecular weight markers (in kDa) is shown

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