Fig. 4

Detection of α3. Immunostaining was conducted on cross- (A, E, I, M, Q and T) and transverse (B, F, J, N, R and U) sections of skeletal muscle. Blots containing reduced and non-reduced sets of lysates from cells expressing α1, α2 and α3 chains individually (C, G, K, O, S and V) were probed for α3. Blots from cell expressing multimeric collagen VI expressing all three chains resolved under non-reducing conditions were probed (D, H, L, and P). Migration position of molecular weight markers (in kDa) is shown between each pair of immunoblots