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Table 1 Summary of collagen VI antibodies and experimental conditions

From: Authentication of collagen VI antibodies

Antibody Supplier Cat number Species Immunogen IHC dilution IB dilution
Collagen VI VI-26 Millipore MAB3303 mou mAb Human-tissue not specified 1:100 1:500
Collagen VI Fitzgerald Ind. 70R-CR009X rab pAb Human/bovine placenta 1:100 1:1000
Collagen VI 172C2 Santa Cruz sc-47764 mou mAb Human-tissue not specified 1:100 1:500
Collagen VI 5C6 U. Iowa DSHB mou mAb Human fetal membranes 1:100 1:500
Collagen VI Millipore AB7821 rab pAb Human placenta 1:100 1:500
COL6A1 H200 Santa Cruz sc-20649 rab pAb α1 chain, amino acid 51-250 1:40 1:500
COL6A1 Abcam ab199720 rab mAb α1 chain, within aa 800 to C-term. 1:400 1:2000
COL6A1 Abcam ab182744 rab mAb α1 chain within amino acid 1-250 1:100 1:1000
COL6A1 Proteintech Gp 17023-1-AP rab pAb Not available 1:100 1:500
COL6A1 B4 Santa Cruz sc-377143 mou mAb α1 chain amino acid 53-86 1:40 1:500
COL6A2 Proteintech Gp 14853-1-AP rab pAb Not available 1:100 1:500
COL6A2 EPR7889 Abcam ab180855 rab mAb α2 chain, amino acid 800-900 1:400 1:1000
COL6A2 Abcam ab172606 rab mAb α2 chain, amino acid 200-300 1:100 1:1000
COL6A2 D20 Santa Cruz sc-83607 rab pAb α2 chain, internal epitope 1:40 1:500
COL6A2 H300 Santa Cruz sc-292186 rab pAb α2 chain, amino acid 241-540 1:40 1:500
COL6A2 K15 Santa Cruz sc-377143 rab pAb α2 chain, C-terminus 1:40 1:500
COL6A2 B7 Santa Cruz sc-374566 mou mAb α2 chain, amino acid 241-540 1:40 1:500
COL6A3 G18 Santa Cruz sc-131139 goat pAb Not available 1:40 1:500
COL6A3 3C4 Millipore MAB1944 mou mAb Not available 1:100 1:1000
COL6A3 3C4 Santa Cruz sc-47712 mou mAb Not available 1:100 1:250
COL6A3 H300 Santa Cruz sc-367543 rab pAb α3 chain, amino acid 301-600 1:40 1:500
COL6A3 H3-2 Santa Cruz sc-81766 mou mAb α3 chain, globular domains 1:100 1:500
COL6A3 64C11 Abcam ab49273 mou mAb α3 chain, 295 aa C-term. frag 1:100 1:1000
COL6A3 N12 Santa Cruz sc-131140 goat pAb Not available 1:40 1:500
  1. Antibodies are grouped by name and clone and each entry includes supplier and catalogue number, species antibody was raised in and whether the antibody is monoclonal or polyclonal. Also listed is the immunogen/epitope used to generate the antibody, if known, and the dilutions used for each application