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Table 1 Summary of collagen VI antibodies and experimental conditions

From: Authentication of collagen VI antibodies

Antibody

Supplier

Cat number

Species

Immunogen

IHC dilution

IB dilution

Collagen VI VI-26

Millipore

MAB3303

mou mAb

Human-tissue not specified

1:100

1:500

Collagen VI

Fitzgerald Ind.

70R-CR009X

rab pAb

Human/bovine placenta

1:100

1:1000

Collagen VI 172C2

Santa Cruz

sc-47764

mou mAb

Human-tissue not specified

1:100

1:500

Collagen VI 5C6

U. Iowa DSHB

–

mou mAb

Human fetal membranes

1:100

1:500

Collagen VI

Millipore

AB7821

rab pAb

Human placenta

1:100

1:500

COL6A1 H200

Santa Cruz

sc-20649

rab pAb

α1 chain, amino acid 51-250

1:40

1:500

COL6A1

Abcam

ab199720

rab mAb

α1 chain, within aa 800 to C-term.

1:400

1:2000

COL6A1

Abcam

ab182744

rab mAb

α1 chain within amino acid 1-250

1:100

1:1000

COL6A1

Proteintech Gp

17023-1-AP

rab pAb

Not available

1:100

1:500

COL6A1 B4

Santa Cruz

sc-377143

mou mAb

α1 chain amino acid 53-86

1:40

1:500

COL6A2

Proteintech Gp

14853-1-AP

rab pAb

Not available

1:100

1:500

COL6A2 EPR7889

Abcam

ab180855

rab mAb

α2 chain, amino acid 800-900

1:400

1:1000

COL6A2

Abcam

ab172606

rab mAb

α2 chain, amino acid 200-300

1:100

1:1000

COL6A2 D20

Santa Cruz

sc-83607

rab pAb

α2 chain, internal epitope

1:40

1:500

COL6A2 H300

Santa Cruz

sc-292186

rab pAb

α2 chain, amino acid 241-540

1:40

1:500

COL6A2 K15

Santa Cruz

sc-377143

rab pAb

α2 chain, C-terminus

1:40

1:500

COL6A2 B7

Santa Cruz

sc-374566

mou mAb

α2 chain, amino acid 241-540

1:40

1:500

COL6A3 G18

Santa Cruz

sc-131139

goat pAb

Not available

1:40

1:500

COL6A3 3C4

Millipore

MAB1944

mou mAb

Not available

1:100

1:1000

COL6A3 3C4

Santa Cruz

sc-47712

mou mAb

Not available

1:100

1:250

COL6A3 H300

Santa Cruz

sc-367543

rab pAb

α3 chain, amino acid 301-600

1:40

1:500

COL6A3 H3-2

Santa Cruz

sc-81766

mou mAb

α3 chain, globular domains

1:100

1:500

COL6A3 64C11

Abcam

ab49273

mou mAb

α3 chain, 295 aa C-term. frag

1:100

1:1000

COL6A3 N12

Santa Cruz

sc-131140

goat pAb

Not available

1:40

1:500

  1. Antibodies are grouped by name and clone and each entry includes supplier and catalogue number, species antibody was raised in and whether the antibody is monoclonal or polyclonal. Also listed is the immunogen/epitope used to generate the antibody, if known, and the dilutions used for each application