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Fig. 1 | BMC Research Notes

Fig. 1

From: Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris

Fig. 1

Cloning of the S-HDAg gene in the vector pPICZαA. a An agarose gel image showing the PCR product. The arrow indicates the position of the PCR product. b An agarose gel image showing the vector pPICZαA. The arrow indicates the position of the vector pPICZαA. c An agarose gel image showing the recombinant vector pPICZαA-S-HDAg isolated from five E. coli colonies (lanes 1–5) cut by XbaI and KpnI. d Diagrammatic map of the recombinant vector pPICZαA-S-HDAg (drawn using the Clone Manager Suite). e A partial sequence of the recombinant vector pPICZαA-S-HDAg including the α-factor, the S-HDAg gene, and His6-tag (using DNA star software). The arrows indicate the positions of the vector pPICZαA and the S-HDAg gene, respectively. M GeneRuler 1 kb plus DNA ladder (Fermentas)

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