Fig. 3From: Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris Purification of S-HDAg-His6-tag fusion protein using nickel affinity chromatography. L indicates protein ladder; 1–9 are eluted fractions which have imidazole concentrations ranging from 72 to 156 mM (the concentration interval between 2 consecutive fractions is about 12 mM). The gel was visualized by silver stainingBack to article page