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Fig. 4 | BMC Research Notes

Fig. 4

From: Revisiting the in vivo GlnR-binding sites at the genome scale in Bacillus subtilis

Fig. 4

Expression of alsT under the dual control of GlnR and TnrA. Strains were grown in minimal medium supplemented with glutamate or glutamine as the sole nitrogen source. Growth (OD600nm) was monitored every 5 min: black lines, wild type; grey lines, ΔglnR; blue lines, ΔtnrA. a Promoter activity (RLU/OD) of a PalsT′-luc transcriptional fusion with the luc reporter gene is indicated: purple line, wild-type; red line, ΔglnR cells; green line, ΔtnrA cells. Strains were grown in the presence of glutamine. For each strain, one representative curve, out of three independent replicates realized, is shown. b Promoter activity (RLU/OD) of a PalsT′-luc transcriptional fusion with the luc reporter gene is indicated: purple line, wild-type; red line, ΔglnR cells; green line, ΔtnrA cells. Strains were grown in the presence of glutamate. For each strain, one representative curve, out of three independent replicates realized, is shown

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