Fig. 3
From: Role of metabolites of cyclophosphamide in cardiotoxicity
Inhibition by NAC of cell cytotoxicity induced by CY metabolites. a Effect of NAC on cytotoxicity of CY metabolites in H9c2 cells after 24-h exposure (mean + SD from 3 to 4 independent experiments conducted in duplicate). b Effect of NAC on cytotoxicity of CY metabolites in H9c2 cells after 48-h exposure (mean + SD from 3 to 4 independent experiments conducted in duplicate). c Effect of NAC on ROS generated by CY metabolites, as shown by fluorescence intensity of DCFH-DA in cells exposed for 15 min to 30 μM HCY or 30 μM acrolein plus NAC. Fluorescence intensity is shown in arbitrary units (mean + SD from three independent experiments). *p < 0.05 compared with samples without NAC