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Fig. 4 | BMC Research Notes

Fig. 4

From: Role of metabolites of cyclophosphamide in cardiotoxicity

Fig. 4

Optical and fluorescence images of H9c2 cells exposed, in presence or absence of NAC, to CY metabolites. ae Optical images of samples after 24 h exposure: living cell nuclei stained by Hoechst 33342 are blue. Induction of apoptosis in H9c2 cells by CY metabolites in presence or absence of NAC: living cell nuclei stained by Hoechst 33342 are blue; apoptotic cells stained by FITC-conjugated probes are green. a Control (unexposed H9c2 cells); b H9c2 cells exposed to 30 μM HCY; c H9c2 cells exposed to 100 μM acrolein. d H9c2 cells exposed to 30 μM HCY with NAC; e H9c2 cells exposed to 100 μM acrolein with NAC. Magnification, ×100; bar 200 μm

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