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Table 1 Genetic configuration of 10 SSR putative functional markers developed for Passiflora alata

From: Report on the development of putative functional SSR and SNP markers in passion fruits

SSR marker code Primer sequences (5′–3′) Annealing temperature (°C) Locus configurationa Observed segregation in the F1 population (N = 30)a Number of alleles
Pa_F-P10A10 F:TGGAATGTGATTTGCATGG
R:TAGGTATTGCTGGCGAAG
55 1 (oo × ao) 1:1 2
Pa_F-P5B01 F:GCGATGAGTGTGCTAGAGG
R:TTCAGACAGCCGAGGAAG
55 1 (ab × cd) 1:1:1:1 4
Pa_F-Contig77 F:AAGCCAAAGTCGGTGTTG
R:TAGCCCAGACAAGGAAGG
60 1 (oo × ao)
2 (oo × ao)
1:1
1:1
2
2
Pa_F + R-Contig114 F:CTTGCCTCTCTCCCTCTC
R:GGGTTTTGGGTTTGACAG
58 1 (ao × bo) 1:1:1:1 4
Pa_F-Contig13 F:CAGAAGGAAAACCAGCAAG
R:CCCCATCATCATCACTTTC
60 1 (ao × bo) 1:1:1:1 4
Pa_F-Contig66 F:TCCCCCATTTTCTCTTCG
R:CGATTCCCGAACAAGTAAG
55 1 (ao × bo) 1:1 2
Pa_F-P7E10 F:TTAATGCCACAGCCCAAC
R:TGACCCAAAATCAAACACC
55 1 (oo × ao) 1:1 2
Pa_F-P8E05 F:GGAAGCAAACACCAAAATC
R:TCGTCGTCATCGAACCTC
55 1 (ao × oo) 1:1:1:1 4
Pa_F + R-Contig80 F:TGTTCAAGCCCATCTTCG
R:GCTCTCGCTCTTGTCGTAG
55 1 (ao × oo)
2 (ao × oo)
3 (oo × ao)
4 (oo × ao)
1:1
1:1
1:1
1:1
2
2
2
2
Pa_F-P6H04 F:GAATAGGGTCAGCAGGAGG
R:GAGTGTGTCATCGGAGTCC
55 1 (oo × ao)
2 (ao × oo)
1:1
1:1
2
2
  1. aAccording to Wu et al. [22]