Table 1 Primer or probe sequence for intratypic differentiation (ITD)
| Specificity | Primer or probe (polarity) | Primer or probe sequence (5′ → 3′) |
|---|---|---|
| Pan-enterovirus |
PCR-1(A) PCR-2 (S) PanEV probe (S) |
GCGATTGTCACCATWAGCAGYCA GGCCCCTGAATGCGGCTAATCC FAM-CCGACTACTTTGGGWGTCCGTGT-BHQ1 |
| Pan-poliovirusa |
panPV/PCR-1(A) panPV/PCR-2(S) panPV Probe21A(A) |
AYRTACATIATYTGRTAIAC CITAITCIMGITTYGAYATG FAM-TGRTTNARIGCRTGICCRTTRTT-BHQ1 |
| Serotype 1a |
seroPV1A(A) seroPV1,2S(S) seroPV1 probe 16A(A) |
ATCATIYTPTCIARPATYTG TGCGIGAYACIACICAYAT FAM-TGCCYAVICCYGIGMIADYGC-BHQ1 |
| Serotype 2 |
seroPV2A (A) seroPV1,2S (S) seroPV2,probe 5S (S) |
AYICCYTCIACIRCICCYTC TGCGIGAYACIACICAYAT FAM-CARGARGCIATGCCCICARGGIATNGG-BHQ1 |
| Serotype 3a |
seroPV3A (A) sero PV 3S (S) seroPV3,probe 11S (S) |
CCCIAIPTGRTCRTTIKPRTC AAYCCITCIRTITTYTAYAC FAM-CCRTAYGTNGGITTRGCVAAYGC-BHQ1 |
| Sabin 1 |
Sab1/PCR-1(A) Sab1/PCR-2(S) Sab1/probe (A) |
CCACTGGCTTCAGTGTTT AGGTCAGATGCTTGAAAGC CY5-TTGCCGCCCCCACCGTTTCACGGA-BHQ3 |
| Sabin 2 |
Sab2/PCR-1(A) Sab2/PCR-2(S) Sab2/probe (S) |
CGGCTTTGTGTCAGGCA CCGTTGAAGGGATTACTAAA FAM-ATTGGTTCCCCCGACTTCCACCAAT-BHQ1 |
| Sabin 3 |
Sab3/PCR-1(A) Sab3/PCR-2(S) Sab3/probe (S) |
TTAGTATCAGGTAAGCTATC AGGGCGCCCTAACTTT ROX-TCACTCCCGAAGCAACAG-BHQ2 |