Table 2 Primer or probe sequence for Sabin vaccine derived poliovirus (VDPV)
Primer specificity | Primer and probe sequences 5′ → 3′ |
|---|---|
S1 VDPV VP1 (Target aa# 99) | Sense CATGCGTGGCCATTATA Anti-sense CAAATTCCATATCAAATCTA VP1 probe FAM-CACCAAGAATAAGGATAAGC-BHQ1 |
S2 VDPV VP1 (Target aa# 143) | Sense GACATGGAGTTCACTTTTG Anti-sense CTCCGGGTGGTATATAC VP1 probe FAM-CATTGATGCAAATAAC-BHQ1 |
S3 VDPV VP1 (Target aa# 285–290) | Sense CATTTACATGAAACCCAAAC Anti-sense TGGTCAAACCTTTCTCAGA VP1 probe FAM-TAGGAACAACTTGGAC-BHQ1 |