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Fig. 1 | BMC Research Notes

Fig. 1

From: A comparative study of digital PCR and real-time qPCR for the detection and quantification of HPV mRNA in sentinel lymph nodes of cervical cancer patients

Fig. 1

Comparision of qPCR and dPCR. a Results of HPV transcript quantification by both methods. Calculated transcript equivalents per µl template (y-axis) are shown for 10 sentinel lymph nodes, a dilution series of SiHa cells and 3 HPV-negative control cell lines (x-axis). Standard deviations are indicated by error bars. b Detection reliability of the above samples by calculation of coefficients of variations. The coefficients of variation are considerably lower in dPCR analyses. This benefit is especially obvious in samples with a low number of templates

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