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Table 1 Oligonucleotides used for amplification with qPCR and dPCR

From: A comparative study of digital PCR and real-time qPCR for the detection and quantification of HPV mRNA in sentinel lymph nodes of cervical cancer patients

Oligonucleotide

Sequence (5′ → 3′)

HPV position

qPCR forward primer

AATGTTTCAGGACCCACAGG

103–122

qPCR reverse primer

CTCACGTCGCAGTAACTGTTG

206–226

dPCR forward primer

TTCGGTTGTGCGTACAAAGC

755–774

dPCR reverse Primer

AGTGTGCCCATTAACAGGTCTTC

799–821

dPCR TaqMan-MGB probe

CACGTAGACATTCGTACTT

778–796

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BMC Research Notes

ISSN: 1756-0500

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