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Fig. 2 | BMC Research Notes

Fig. 2

From: Donor variability may mask dimethyl fumarate’s effects on nuclear factor E2-related factor 2 in human peripheral blood mononuclear cells

Fig. 2

Dimethyl fumarate has donor-dependent effects on Nrf2 in human peripheral blood mononuclear cells (PBMCs). Analyses presented in this figure are derived from the data presented in Fig. 1 experiments, combining donors from Fig. 1a, c. a Untreated (UNT) values are compared to the maximum Nrf2 activation (OD450) for all donors (Cmax). n = 12, 11 allogeneic. b Donor values for UNT, 2 h DMF and 4 h DMF were analyzed in aggregate (all donors), or broken into groups based on Nrf2 response to DMF treatment. Non-responders: Nrf2 decreases with DMF treatment. Moderate responders: Nrf2 increases modestly at 2 h but drops below UNT by 4 h. Sustained responders: Nrf2 robustly activated at 2 h, remains above UNT at 4 h. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots; crosses represent sample means. n = 12, 12, 11, 3, 3, 2, 4, 4, 4, 5, 5, 5 sample points. This graph was generated by BoxPlotR: a web-tool for generation of box plots. c Graph representing the direction and magnitude of Nrf2 activation status with DMF treatment. Percent change was calculated by subtracting UNT values from the value at the time point at which Nrf2 showed the largest change in activation from compared to UNT (whether that was an increase or decrease) for each donor. NR non-responder, MR moderate responder, SR sustained responder. d Nrf2 results for the same donor repeated in both the Fig. 1a, c experiments demonstrate consistency of donor response to DMF. This donor is a sustained responder. In all panels, *indicates p < 0.05 by Student’s t-test. ANOVA was used prior to t-tests in B and C

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