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Table 1 Viability, yield and purity of isolated hAECs using different protocols

From: Method and key points for isolation of human amniotic epithelial cells with high yield, viability and purity

Article name Authors Published year Viability (%) Yield (per placenta) Percentage of cells with epithelial cell marker Percentage of cells with stem cell markers
Isolation of human amnion epithelial cells according to current good manufacturing procedures Roberto Gramignoli et al. 2016 94.4 ± 0.3% 80–300 × 106 (> 107 cells/g of processed tissue) > 85% CD49f and EpCAM positive cells < 15% CD105 positive cells
Isolation, cryopreservation and culture of human amnion epithelial cells for clinical applications Sean V. Murphy et al. 2014 83 ± 4% 120 ± 40 × 106 92% Ep-CAM positive cells < 1% CD90 & CD105 positive cells
Isolation and Partial characterization of human amniotic epithelial cells: the effect of trypsin Meraj Tabatabaei et al. 2014 > 98% 80–130 × 106 > 99% cytokeratin positive cells > 56% CD105 positive cells
Amnion epithelial cell isolation and characterization for clinical use Sean Murphy et al. 2010 83 ± 4% 120 ± 40 × 106 92% Ep-CAM positive cells < 1% CD90 & CD105 positive cells
Isolation of amniotic epithelial stem cells Toshio Miki et al. 2010 Not reported 80–300 × 106 Not reported 8.79 ± 2.84% SSEA-3 positive cells, 43.94 ± 14.8% SSEA-4 positive cells, 9.82 ± 4.31% TRA 1-60 positive cells & 9.91 ± 4.49% TRA 1-81 positive cells
Human amniotic epithelial cells: isolation and characterization Ruth Gomez Dominguez 2008 Not reported 12 × 106 97% cytokeratin 19 positive cells 30% CD105 positive cells
The potential of amniotic membrane/amnion-derived cells for regeneration of various tissues Ayaka Toda et al. 2007 Not reported 8–50 × 106 Not reported Not reported