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Table 3 Used antibodies to determine phenotypic characterictics of hAECs by flow cytometry

From: Method and key points for isolation of human amniotic epithelial cells with high yield, viability and purity

Primary antibodies/ fluorochrome

Isotype

Catalog number

Source of primary antibodies

Alexa Fluor® 488 anti-Cytokeratin (pan reactive)

Mouse IgG1, κ (cat. no:400143)

628608

Biolegend, San Diego, CA, USA

FITC anti-human CD105

Mouse IgG1, κ (cat. no:400107)

323203

Biolegend, San Diego, CA, USA

FITC anti-human CD90

Mouse IgG1, κ (cat. no:400107)

328107

Biolegend, San Diego, CA, USA

FITC anti-human CD45

Mouse IgG1, κ (cat. no:400107)

368507

Biolegend, San Diego, CA, USA

FITC anti-human CD14

Mouse IgG1, κ (cat. no:400107)

367115

Biolegend, San Diego, CA, USA

FITC anti-human CD4

Mouse IgG1, κ (cat. no:400107)

357405

Biolegend, San Diego, CA, USA

FITC anti-human CD8a

Mouse IgG1, κ (cat. no:400107)

300905

Biolegend, San Diego, CA, USA

PE anti-human CD56

Mouse IgG1, κ (cat. no:400111)

355503

Biolegend, San Diego, CA, USA

FITC anti-human CD3

Mouse IgG1, κ (cat. no:400107)

362305

Biolegend, San Diego, CA, USA

FITC mouse anti-human HLA-DR

Mouse IgG2a, κ(cat. no: 555057)

555560

BD Biosciences, San Jose, CA, USA

FITC mouse anti-human CD34

Mouse IgG1, κ(cat. no: 555748)

555821

BD Biosciences, San Jose, CA, USA

FITC mouse anti-human CD38

Mouse IgG1, κ(cat. no: 555748)

555459

BD Biosciences, San Jose, CA, USA

PE mouse anti-human CD44

Mouse IgG1, κ(cat. no: 550617)

550989

BD Biosciences, San Jose, CA, USA

PE mouse anti-human CD9

Mouse IgG1, κ(cat. no: 550617)

555372

BD Biosciences, San Jose, CA, USA

PE mouse anti-human CD29

Mouse IgG1, κ(cat. no: 550617)

557332

BD Biosciences, San Jose, CA, USA

PE mouse anti-human CD73

Mouse IgG1, κ(cat. no: 550617)

550257

BD Biosciences, San Jose, CA, USA

Anti-human SSAE-4 PE

Mouse/ IgG3(cat. no:12-4742-42)

12-8843-42

Thermo Fisher Scientific, Waltham, MA, USA

Anti-human CD133 FITC

Mouse/ IgG2b, kappa(cat. no: 11-4732-42)

11-1339-42

Thermo Fisher Scientific, Waltham, MA, USA