Skip to main content

Advertisement

Table 3 Used antibodies to determine phenotypic characterictics of hAECs by flow cytometry

From: Method and key points for isolation of human amniotic epithelial cells with high yield, viability and purity

Primary antibodies/ fluorochrome Isotype Catalog number Source of primary antibodies
Alexa Fluor® 488 anti-Cytokeratin (pan reactive) Mouse IgG1, κ (cat. no:400143) 628608 Biolegend, San Diego, CA, USA
FITC anti-human CD105 Mouse IgG1, κ (cat. no:400107) 323203 Biolegend, San Diego, CA, USA
FITC anti-human CD90 Mouse IgG1, κ (cat. no:400107) 328107 Biolegend, San Diego, CA, USA
FITC anti-human CD45 Mouse IgG1, κ (cat. no:400107) 368507 Biolegend, San Diego, CA, USA
FITC anti-human CD14 Mouse IgG1, κ (cat. no:400107) 367115 Biolegend, San Diego, CA, USA
FITC anti-human CD4 Mouse IgG1, κ (cat. no:400107) 357405 Biolegend, San Diego, CA, USA
FITC anti-human CD8a Mouse IgG1, κ (cat. no:400107) 300905 Biolegend, San Diego, CA, USA
PE anti-human CD56 Mouse IgG1, κ (cat. no:400111) 355503 Biolegend, San Diego, CA, USA
FITC anti-human CD3 Mouse IgG1, κ (cat. no:400107) 362305 Biolegend, San Diego, CA, USA
FITC mouse anti-human HLA-DR Mouse IgG2a, κ(cat. no: 555057) 555560 BD Biosciences, San Jose, CA, USA
FITC mouse anti-human CD34 Mouse IgG1, κ(cat. no: 555748) 555821 BD Biosciences, San Jose, CA, USA
FITC mouse anti-human CD38 Mouse IgG1, κ(cat. no: 555748) 555459 BD Biosciences, San Jose, CA, USA
PE mouse anti-human CD44 Mouse IgG1, κ(cat. no: 550617) 550989 BD Biosciences, San Jose, CA, USA
PE mouse anti-human CD9 Mouse IgG1, κ(cat. no: 550617) 555372 BD Biosciences, San Jose, CA, USA
PE mouse anti-human CD29 Mouse IgG1, κ(cat. no: 550617) 557332 BD Biosciences, San Jose, CA, USA
PE mouse anti-human CD73 Mouse IgG1, κ(cat. no: 550617) 550257 BD Biosciences, San Jose, CA, USA
Anti-human SSAE-4 PE Mouse/ IgG3(cat. no:12-4742-42) 12-8843-42 Thermo Fisher Scientific, Waltham, MA, USA
Anti-human CD133 FITC Mouse/ IgG2b, kappa(cat. no: 11-4732-42) 11-1339-42 Thermo Fisher Scientific, Waltham, MA, USA