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Table 1 In-house ELISAs

From: Effects of freezer storage time on levels of complement biomarkers

Analyte

Capture antibody

Detection antibody

Standard curve

Plasma dilution

sCR1

1 µg/ml RP anti-CR1

1 µg/ml MM HRP labelled anti-human CR1

50, 25, 12.5, 6.25, 3.125, 1.565, 0.78, 0 ng/ml

1:2

C5

1 µg/ml RP anti-C5

1 µg/ml MM HRP labelled anti-human C5

1000, 500, 250, 125, 62.5, 31.25, 15.625, 0 ng/ml

1:100

C9

1 µg/ml MM anti-C9

1 µg/ml RP HRP labelled anti-human C9

100, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0 ng/ml

1:2000

C1q

2 µg/ml MM anti-C1q (commercial WL02 from Hycult)

1 µg/ml RP HRP labelled anti-human C1q

1000, 500, 250, 125, 62.5, 31.25, 15.625, 0 ng/ml

1:800

C1 inhibitor

1 µg/ml MM anti-C1 inhibitor

1 µg/ml RP HRP labelled anti-human C1 inhibitor

100, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0 ng/ml

1:16,000

  1. MM mouse monoclonal antibody, RP rabbit polyclonal antibody