TY - JOUR AU - Stieber, Bettina AU - Sabat, Artur AU - Monecke, Stefan AU - Slickers, Peter AU - Akkerboom, Viktoria AU - Müller, Elke AU - Friedrich, Alexander W. AU - Ehricht, Ralf PY - 2017 DA - 2017/11/07 TI - PVL overexpression due to genomic rearrangements and mutations in the S. aureus reference strain ATCC25923 JO - BMC Research Notes SP - 576 VL - 10 IS - 1 AB - ATCC25923 is a Staphylococcus aureus strain that is positive for the Panton Valentin leukocidin. It has been used for decades as reference strain. We observed that two separately maintained clones of ATCC25923 (“G477 and G478”) differed grossly in the expression of this toxin. For that reason, both clones were sequenced using an Illumina MiSeq instrument. After assembling, the final sequences were analyzed and mapped to a previously published ATCC25923 sequence (GenBank CP009361) using bl2seq from the NCBI Blast2 package. SN - 1756-0500 UR - https://doi.org/10.1186/s13104-017-2891-3 DO - 10.1186/s13104-017-2891-3 ID - Stieber2017 ER -