Fig. 4
From: Evaluation of the effect of a floxed Neo cassette within the dystroglycan (Dag1) gene
Western blot analysis. Representative immunoblotting of total protein lysates from Dag1 +/+, Dag1 Neo/+, Dag1 Neo/Neo, and Dag1 ΔNeo/ΔNeo skeletal muscle. Upper panel; α-DG probed with IIH6 antibody specific for the α-DG carbohydrates epitopes; middle panel: tubulin used as loading control; lower panel: β-DG probed with the anti 43-DAG antibody showing the correct processing of the DG precursor