Fig. 6

Quantitative analysis of Dag1 expression in skeletal muscle of 5 months old Dag1 ^+/+, Dag1 ^Neo/+, Dag1 ^Neo/Neo, and Dag1 ^ΔNeo/ΔNeo mice. Total mRNA was purified from tibialis anterior skeletal muscle of 5 months old mice and retro-transcribed to cDNA. Real-Time PCR reactions were performed using probes for the Dag1 and Gadph genes. Results are expressed as fold change of Dag1 expression in Dag1 ^Neo/+ (n = 4), Dag1 ^Neo/Neo (n = 4), and Dag1 ^ΔNeo/ΔNeo (n = 4) lines relative to the Dag ^+/+ line (n = 4). No significant difference was noted between any of the genotypes (p = 0.707). Error bars represent SEM