Marker
|
Msh2-Msh3
+/+
|
Msh2-Msh3
GD/GD
|
Msh2-Msh3
−/−
|
---|
Somatic MSI
|
U12335(A)n
|
(5/95) 5%
|
(16/72) 22%
|
(1/99) 1%
|
TG27
|
(3/90) 3%
|
(8/95) 8%
|
(11/75) 15%
|
D7Mit91
|
(0/77) 0%
|
(1/77) 1%
|
(7/79) 9%
|
Tumor MSI
|
U12335
|
(0/6) 0%
|
(4/8) 50%
|
(2/15) 13%
|
TG27
|
(0/6) 0%
|
(1/8) 13%
|
(4/8) 50%
|
- Somatic and tumor microsatellite instability: MSI was analyzed using the mononucleotide marker U12235n and dinucleotide markers TG27 and D7Mit91 using pooled tail DNA from five each, Msh2-Msh3
+/+
, Msh2-Msh3
−/−
and Msh2-Msh3
GD/GD mice, (p values U12335: Msh3+/+ vs. Msh3GD/GD = 0.036 and Msh3+/+: Msh3−/− = 0.02, p values TG27: Msh3+/+ vs. Msh3GD/GD = 0.06 and Msh3+/+ vs. Msh3−/− = 0.038, p values D7Mit91: Msh3+/+ vs. Msh3−/− = 0.05)