Table 1 Forward and reverse primer specifications for AT2G40420 promoter sequence
Primer | Primer sequence (5′→3′) | Tm °C | GC% | Product size |
|---|---|---|---|---|
AT2G40420F | CCTACTAGTTCGTGATACTG | 52.05 | 45.00 | 1781 bp |
AT2G40420R | CGAACGATTCCTTCATCACG | 57.02 | 50.00 |
Primer | Primer sequence (5′→3′) | Tm °C | GC% | Product size |
|---|---|---|---|---|
AT2G40420F | CCTACTAGTTCGTGATACTG | 52.05 | 45.00 | 1781 bp |
AT2G40420R | CGAACGATTCCTTCATCACG | 57.02 | 50.00 |