Fig. 1

Comparison of Cq values from RT and No-RT samples. Three of the seven primer/probe sets detected signals from No-RT controls (n = 8–10) in qPCR including Actb, Gapdh and Ywhaz. When the Cq values of No-RT controls were compared to those of RT samples (RNA samples went through reverse transcription step), significant larger values of No-RT controls was observed for Actb and Ywhaz, but not for Gapdh